Transaminase Activity in Neurospora crassa

Transaminase Activity in Neurospora crassa

It is now believed that the amino groups of isoleucine and valine are accepted by their respective carbon chains during biosynthesis via a transamination. Most of the evidence for this hypothesis has been derived from experiments on isoleucineless and valineless mutants of Escherichia coli and Neurospora crassu (l-4). It is not positively established whether this transamination step can be medi...

Adenylate cyclase activity in Neurospora crassa.

Adenylate cyclase, the enzyme responsible for cyclic adenylate synthesis has been found in membrane fractions of animal cells (l-4). This enzyme system is stimulated by several hormones, showing a wide range of specificity. The occurrence of a similar enzyme activity has been also demonstrated in Escherichia coli and Bacillus liquejaciens (5-7)) but in these organisms the mechanism that regulat...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...